Li C
China
Research Article
Preparative Separation of Lappaconitine from Aconitum leucostomum by HSCCC
Author(s): Amatjan A, Slukhan U, Li C, Ke-Lin HU and Haji Akber AisaAmatjan A, Slukhan U, Li C, Ke-Lin HU and Haji Akber Aisa
This paper describes a system for the preparation of lappaconitine from Aconitum leucostmum Worosch., using HSCCC, and examines the effect of the two-immiscible solvent system (TISS) composed of chloroform- methanol-0.2 mol/L hydrochloric acid (4:1.5:2). Under the optimized condition, about 75 mg of lappaconitine, at a purity of 98% that was done by high performance liquid chromatography (HPLC), are obtained from 250 mg of the crude alkaloid extract. The compound was checked by HPLC-UV, electrospray ionization mass spectrometry (ESI-MS), 1H NMR and 13C NMR.
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