Haji Akber Aisa
Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, the Key Laboratory of Xinjiang Indigenous, Medicinal Plants Resource Utilization,
Urumqi 830011
China
Research Article
Preparative Separation of Lappaconitine from Aconitum leucostomum by HSCCC
Author(s): Amatjan A, Slukhan U, Li C, Ke-Lin HU and Haji Akber AisaAmatjan A, Slukhan U, Li C, Ke-Lin HU and Haji Akber Aisa
This paper describes a system for the preparation of lappaconitine from Aconitum leucostmum Worosch., using HSCCC, and examines the effect of the two-immiscible solvent system (TISS) composed of chloroform- methanol-0.2 mol/L hydrochloric acid (4:1.5:2). Under the optimized condition, about 75 mg of lappaconitine, at a purity of 98% that was done by high performance liquid chromatography (HPLC), are obtained from 250 mg of the crude alkaloid extract. The compound was checked by HPLC-UV, electrospray ionization mass spectrometry (ESI-MS), 1H NMR and 13C NMR.
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