Takuhiro Uto
Japan
Research Article
Interaction Analysis of Glycyrrhizin on Licorice Extract-Induced Apoptosis of Human Leukemia Cells by Knockout Extract
Author(s): Takuhiro Uto, Nguyen Huu Tung, Osamu Morinaga and Yukihiro ShoyamaTakuhiro Uto, Nguyen Huu Tung, Osamu Morinaga and Yukihiro Shoyama
Licorice is the most frequently prescribed herb in traditional Chinese medicine (TCM) and Japanese Kampo medicine. Although glycyrrhizin (GC) is one of the major active components of licorice, the other components are also known to possess biological activities. We had previously prepared GC-knockout (GC-KO) extract, which contained all components of licorice extract (LE) except GC using a immunoaffinity column conjugated with anti-GC monoclonal antibodies (MAb). In this study, we used the GC-KO extract to investigate the potential role of GC in LE-induced apoptotic cell death. LE markedly induced apoptotic cell death in human leukemia HL-60 cells. Although treatment with GC alone had no influence on cell viability, induction of cell death by the GC-KO extract was weaker than that by LE. Interestingly, the addition of GC to the GC-KO extract significantly rescued cell grow.. View More»
