Shiping Liu
Institute of Metabolism and Endocrinology, Second Xiangya Hospital,
Changsha, Hunan 410011
China
Research Article
Obese Patient-Derived Sera Have Proinflammatory Effect
Author(s): Lan Yao, Kuan Yan, Yang Xiao, Aimin Xu, Zhiguang Zhou and Shiping LiuLan Yao, Kuan Yan, Yang Xiao, Aimin Xu, Zhiguang Zhou and Shiping Liu
Aim: Chronic inflammation of
adipose tissues
plays a key role in obesity-induced metabolic disorders. We examined effects of sera from obese patients with or without different compositions of metabolic disorders on the activation of Toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway in the human monocytic leukemia cell line (THP-1), which have never been reported. Methods: With or without pretreatment of TLR4 monoclonal antibody, THP-1 cells were incubated for 48h by sera from 45 obese patients with or without metabolic disorders and 15 controls. The level of TLR4 in THP-1 cells, intracellular level of phosphorylated NF-κB p65, IL-1β and TNF-α levels in cell culture supernatants were measured. Results: Compared with controls, the expression level of TLR4, NF-κB p65, IL-1β and TNF-α were significantly.. View More»
