Sanam Zeib Khan
Pakistan
Research Article
18bp Fragment Insertion/Deletion Polymorphism of Vascular Endothelial Growth Factor (VEGF) Gene with Diabetes Mellitus Type 2 and Diabetic Retinopathy Patients of Quetta, Balochistan, Pakistan
Author(s): Sanam Zeib Khan, Rozeena Shaikh, Muhammad Azhar, Abdul Wali and Jamil AhmadSanam Zeib Khan, Rozeena Shaikh, Muhammad Azhar, Abdul Wali and Jamil Ahmad
Background: An important candidate gene for diabetic retinopathy (DR) is vascular endothelial growth factor (VEGF). The VEGF gene is extremely polymorphic. The 18 base pairs (bp) segment (I/D) polymorphism at -2549 position of the promoter region is of great importance.
Aim: The present study aimed to identify VEGF (I/D) polymorphism in DM and DR patients.
Materials and Methods: This cross section study involved 100 healthy control subjects, 100 diabetes mellitus (DM) subjects and 100 DR subjects. Blood samples were collected after informed consent of study subjects, DNA extraction was performed using inorganic method, VEGF gene promoter region that was confirmed using 2% agarose gel. Polymerase Chain Reaction (PCR) was used to identify (I/D) polymorphism of 18 bp fragment at position -2549, DNA Sequencing was.. View More»
