Rajni Rani
Molecular Immunogenetics Group, National Institute of Immunology, Aruna Asaf Ali Marg,
New Delhi-110067
India
Research Article
Haplotypes of Polymorphic Antigen Processing Genes for Low Molecular Mass Polypeptides (LMP2 and LMP7) are Strongly Associated with Type 1 Diabetes in North India
Author(s): Bhukya Saida, Prachi Dani, Nachiketa Patnaik, Bharti Agrawal, T Rajaratna, Anushree Jaiswal, Alok Kumar Singh and Rajni RaniBhukya Saida, Prachi Dani, Nachiketa Patnaik, Bharti Agrawal, T Rajaratna, Anushree Jaiswal, Alok Kumar Singh and Rajni Rani
Objective: Type 1 diabetes (T1D) is a multifactorial autoimmune disorder where several genes have been
implicated. Aberrant presentation of auto-antigens on the MHC molecules is the hallmark of autoimmune disorders.
The antigen is processed into small peptides by low molecular mass polypeptides, LMP2 and LMP7 before they are
presented on the MHC class-I molecules. We have studied the associations of the Single nucleotide polymorphisms
(SNPs) in these antigen processing genes and their haplotypes which may be detrimental for the processing of the
auto-antigens in T1D.
Methods: 239 T1D subjects and 752 normal healthy controls from North India were studied for LMP2 codon
60 G/A (R/H), LMP7 codon 49 of C/A (Q/K) and LMP7 Intron 6 G/T polymorphisms using PCR-RFLP. HLA class-II
alleles were studied using bead based Luminex assays. Hapl.. View More»
