Peter Almgren
Sweden
Research Article
The Rhesus Monkey: A Nonhuman Primate Model For T2DM- Associated Gene Screening
Author(s): Barbara C. Hansen, Rania Shamekh, Ola Hansson, Peter Almgren, Temuri Budagov, Ellen Linden, Jeffrey Pessin and Gil AtzmonBarbara C. Hansen, Rania Shamekh, Ola Hansson, Peter Almgren, Temuri Budagov, Ellen Linden, Jeffrey Pessin and Gil Atzmon
To examine the genetic basis of the spontaneous development of Type 2 diabetes mellitus (T2DM), we have conducted an initial genome wide association study (GWAS) using Affymetrix 6.0 gene chip arrays on 8 normal (Controls), and 14 insulin resistant(IR)/prediabetic(preDM)/T2DM rhesus monkeys ( Macaca mulatta ). Both IR/preDM and T2DM were classified as cases as they could be clearly defined in this model as either in the early progression toward overt DM or already overtly diabetic. The successful call rate on all chip assays averaged 91.9% with high heterozygosity (42%), demonstrating the project applicability for genotyping the rhesus monkey using the human platform. Principal component analysis classified the groups accurately according to their source – i.e. case or control. Five SNPs (Chr 18, and 1 of the human and rhesus genomes) passed the tests for multiple comparison cor.. View More»
