Pedersen BK
Centre of Inflammation and Metabolism,
Rigshospitalet- Section 7641, Blegdamsvej 9, DK-2100, Copenhagen
Denmark
Research Article
Altered Subcutaneous Adipose Tissue Response to Systemic LPS Administration in Patients with Type 2 Diabetes
Author(s): Mathur N, Andreasen AS, Berg RMG, Pedersen M, Møller K, Pedersen BK and Laye MJMathur N, Andreasen AS, Berg RMG, Pedersen M, Møller K, Pedersen BK and Laye MJ
Aim: Inflammation is thought to play a major role in impaired metabolism. However, the metabolic and inflammatory response of adipose tissue, to a pro-inflammatory stimulus is poorly defined in patients with Type 2 Diabetes Mellitus (T2DM). We therefore aimed to investigate whether adipose tissue in T2DM would display an altered response to E. coli LipoPolySaccharide (LPS).
Materials and methods: Twelve patients with T2DM and 12 control subjects received an intravenous bolus injection of LPS (0.3 ng/kg). Abdominal subcutaneous adipose tissue biopsies, serum and plasma were obtained at 0, 2, 4, 6 and 8 hours after LPS. The gene expression of Tumour Necrosis Factor-α (TNF), InterLeukin-6 (IL-6), lipoprotein lipase (LPL), hormone sensitive lipase (HSL), fatty acid synthase (FASN), adiponectin and peroxisome proliferator-activated recptor γ (PPARγ) was analysed by.. View More»
