Makiko Kumagai-Braesch
CLINTEC, Karolinska Institutet Stockholm,
Sweden
Research Article
An Engineered Innate Repair Receptor Agonist, ARA 290, Protects Rat Islets from Cytokine-induced Apoptosis
Author(s): Masaaki Watanabe, Yu Saito, Jesper Wallmo, Tohru Takahashi, Randa A Hadi Diab, Ming Han Yao, Michael Brines, Anthony Cerami, Claes- Goran Ostenson, Torbjorn Lundgren and Makiko Kumagai-BraeschMasaaki Watanabe, Yu Saito, Jesper Wallmo, Tohru Takahashi, Randa A Hadi Diab, Ming Han Yao, Michael Brines, Anthony Cerami, Claes- Goran Ostenson, Torbjorn Lundgren and Makiko Kumagai-Braesch
The efficacy of pancreatic islet transplantation (PITx) is reduced due to severe inflammatory responses triggered by the islet isolation and transplantation procedures. ARA 290, an innate repair receptor agonist, has anti-apoptotic and anti-inflammatory properties and it has been shown to improve clinical symptoms caused by inflammation in sarcoidosis patients and type 2 diabetes. We here investigated whether ARA 290 treatment would improve the efficacy of PITx in a rat pancreatic islet transplantation model. For islets co-cultured with proinflammatory cytokines, addition of ARA290 showed better viability [percent of naïve control in MTT assay: 54.6 ± 5.2 vs. 75.2 ± 6.4], insulin release [stimulation index in static glucose stimulated insulin secretion tests of 1.05 ± 0.63 vs. 2.61 ± 0.89], and reduced.. View More»
