Iván Sergio CG
Natural Products Laboratory (Lapron),
Brazil
Research Article
Periconia hispidula A New Semiarid Biocatalyst for the Reduction of Prochiral Aromatic Ketone
Author(s): Serly S, Luis Fernando PG, Angelica L, Heiddy MA and Iván Sergio CGSerly S, Luis Fernando PG, Angelica L, Heiddy MA and Iván Sergio CG
The ability of fungus Periconia hispidula (42/07), isolated at the semi-arid of BAHIA in Brazil, to biotransform acetophenone and its derivatives has been studied. The influence of reaction parameters (mean, time, amount of substrate) on the conversion and enantioselectivity of acetophenone reduction was initially investigated. By comparison between culture media (optimized YM / BD / BD supplemented with Fe2+) BD medium supplemented with Fe2+ (0.02 g/L) was selected. From the growth curve, the incubation time prior to addition of substrate (4 and 7 days) was determined. The best conversion results for obtaining the (S)-1-phenylethanol, with enantiomeric excess of 98% were reached after four days of previous incubation of the microorganism. The influence of the amount of acetophenone (20 μL to 200 μL) was also analyzed with the objective of evaluating the toxicity of the substrat.. View More»
