Hassanali A
Kenya
Research Article
Biflavonoids from an Ethno-Medicinal Plant Ochna holtzii Gilg
Author(s): Awadh MM, Tarus PK, Onani MO, Machocho AK and Hassanali AAwadh MM, Tarus PK, Onani MO, Machocho AK and Hassanali A
Ochna holtzii Gilg is a medicinal plant used extensively at the Kenya Coast for the treatment of various ailments. From solvent extracts of the root and stem barks of O. holtzii, seven constituents were isolated by standard chromatographic techniques (CC, VLC, prep-TLC and Sephadex LH-20). Their structures were analyzed by MS, UV,IR,1D and 2D NMR spectroscopy. All were found to be biflavonoids, including three novel compounds: dehydrate of lophirone C, hotzinol, and tri-O-methyl lophirone A. Crude methanol extracts of O. holtzii and the isolated biflavonoids were tested for antimicrobial activities against two Gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis, two Gram-negative bacteria, Salmonella typhi and Pseudomonas aeruginosa, and the diploid fungus Candida albicans. The extracts and the isolated constituents sh.. View More»
