Hana M Hammad
Jordan
Research Article
Phytochemical Analysis and Evaluation of Anti-angiogenic and Antiproliferative Activities of the Leaves of Elaeagnus angustifolia L. Grown in Jordan
Author(s): Aman A Ishaqat, Rana Abu-Dahab, Hana M Hammad, Malek Al-Zihlif, Ismail F Abaza, Zeyad D Nassar and Fatma U AfifiAman A Ishaqat, Rana Abu-Dahab, Hana M Hammad, Malek Al-Zihlif, Ismail F Abaza, Zeyad D Nassar and Fatma U Afifi
Elaeagnus angustifolia L. has a long history of use in ethnopharmacology. Only few studies examined the potential activities of the leaves. Furthermore, the leaves’ chemical composition was not fully investigated. In this study, the chemical composition of E. angustifolia leaves extract was analysed and major compounds were isolated and identified. Extract obtained by maceration was further extracted with solvents differing in their polarity then submitted to open column chromatography, followed by isolation of major compounds. They were analysed using UV-Vis and/or NMR. One terpene (β-sitosterol) and four flavonoids (chrysin-7-glucoside, rutin, luteolin and kaempferol) were isolated and identified. For the biological activities, leaves were extracted using ethanol, ethyl acetate, chloroform and water. Anti-angiogenic activity was studied by rat aortic rin.. View More»
