Gupta SK
India
Research Article
Differential Expression of Toll like Receptor 4 in Type 2 Diabetic Patients with Impaired Wound Healing
Author(s): Kanhaiya, Agrawal NK, Gupta SK and Kiran SinghKanhaiya, Agrawal NK, Gupta SK and Kiran Singh
Toll like Receptor 4 (TLR4), is known for its key role in initiation of innate immunity and regulation of adaptive immune responses. TLR4 is also an important regulator of wound inflammation, stimulator of growth factors like vascular endothelial growth factor (VEGF) and plays an important role in restoring damaged tissue integrity during normal wound healing. In the present study we have investigated 78 Diabetic Foot ulcer (DFU) patients with Type 2 diabetes (T2DM) and 8 foot ulcers patients without T2DM as controls for the differential expression of TLR4 both at mRNA level and protein level. Expression analysis was done using semi-quantitative RT-PCR, quantitative Real-time PCR and western blot. TLR4 message and protein were significantly down-regulated in DFU patients as compared to controls. Similarly down regulation of VEGF was also observed in DFU patients as compared to control.. View More»
