Elizabeth Shea
Tanzania
Research Article
Rate of Formation of Glycated Albumin Revisited and Clinical Implications
Author(s): Margo P. Cohen, Elizabeth Hud and Elizabeth SheaMargo P. Cohen, Elizabeth Hud and Elizabeth Shea
Background: Albumin modified by Amadori glucose adducts contributes to the pathogenesis of complications of diabetes and reducing its formation ameliorates their development, underscoring the need for accurate information on the rate of formation of this biologically active glycated protein. However, this subject has not been examined in over two decades, and there is reason to question data in older reports. Methods: The present study used nonradioactive and non reductive techniques to examine the rate of formation of glycated albumin and compare it to that of glycohemoglobin, using a sensitive and specifi c immunoassay for measurement of the stable glucose adduct formed after incubation of serum, plasma and purified albumin with glucose. Results and Conclusions: We report that the rate of formation of glycated albumin parallels that of glycohemoglobin at approximately 0.005-0.008 pe.. View More»
