Bikash Debnath
India
Research Article
Phytochemical Analysis, Anti-oxidant and Cytotoxic Activity of Seed Coat of Macrotyloma uniflorum in Different Solvents
Author(s): Gayatri Chakraborty, Kuntal Manna, Bikash Debnath, Waikhom Somraj Singh, Sanchari Goswami and Debasish MaitiGayatri Chakraborty, Kuntal Manna, Bikash Debnath, Waikhom Somraj Singh, Sanchari Goswami and Debasish Maiti
In this study, the determination of secondary metabolites, anti-oxidant and cytotoxic activity of seed coat of Macrotyloma uniflorum were carried out in different solvents (petroleum ether, chloroform, ethanol, and water), according to their increasing polarity. Alkaloid and tannin content was analyzed by simple titrimetric method. Colorimetric method was used for the determination of phenolic, flavonoid, terpenoid and saponin content. Antioxidant activity was determined by DPPH free radical scavenging assay and cytotoxic activity was evaluated by Trypan blue exclusion assay. The results indicated that sample seed coat extracted with ethanol (sample 3) contained high level of alkaloid (0.66 ± 0.01 mg/g), phenol (42.38 ± 0.53 mg/g), flavonoid (22.81 ± 0.48 mg/g), tannin (55.89 ± 0.75 mg/g), saponin (24.85 ± 0.53 mg/g), and terpenoid (54.60 &p.. View More»
