Ayse A
Turkey
Research Article
Extraction and HPLC Analysis of Sage (Salvia officinalis) Plant
Author(s): Fatma Ebru K, Ayse A and Caglar KFatma Ebru K, Ayse A and Caglar K
In this study, the sage (Salvia officinalis) plant collected from the Mediterranean region was extracted with different solvents and methods. The extract yields were compared. The quantities of rosmarinic acid, carnosic acid and carnosol, which are responsible for the antioxidant capacity of the sage plant, were analyzed qualitatively and quantitatively by an HPLC.
Ground sage plant were sieved with 70 mesh sieves. Maceration were done for powder sage plant at 45°C with 70% ethyl alcohol in “1:6” (w/v) ratio at different time (3; 6; 8; 10 hours); 100% methanol and 100% ethanol extractions; Soxhlet extraction efficiencies were compared. The proportions of rosmarinic acid and the amounts of carnosic acid and carnosol were analyzed in the extracted at UV detector and 280 nm wavelength by a Thermo Scientific Ulimate HPLC instrument. As a result of the experiments, .. View More»
