Anita Surendra Patil
Amravati
India
Research Article
Evaluation of Effect of Gamma Radiation on Total Phenolic Content, Flavonoid and Antioxidant Activity of In Vitro Callus Culture of Artemisia annua
Author(s): Anita Surendra Patil, Pooja Suryavanshi and Devanand FulzeleAnita Surendra Patil, Pooja Suryavanshi and Devanand Fulzele
Gamma irradiations are physically induced stresses that has been considered as the rapid and fast method to enhance the quality and quantity of plant characteristics. Phenolic compounds and flavonoids possess a wide range of biological activities such as antioxidant, anti-inflammatory, anti-aging, anti-bacterial and anti-tumor functions and have high economical and pharmaceutical values. In current study In vitro callus cultures were exposed to gamma irradiation at different doses 5, 10, 15, 20, 25, 30 and 35 Gy. After 25 days of gamma irradiation the gamma irradiated callus (M0T0) culture were further subcultured on 0.5 mg/L napthalein acetic acid (NAA) and benzyl amino purine (BAP) till M5T5 generation. The free radical scavenging activity of gamma irradiated methanolic callus extract was measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method. The highest free radical sca.. View More»
