Akihiro Kudoh
Japan
Research Article
Resistin Exacerbates Insulin Resistance under the Condition of Low Adiponectin in 3T3-L1 Adipocytes
Author(s): Noritaka Machii, Hiroaki Satoh, Akihiro Kudoh and Tsuyoshi WatanabeNoritaka Machii, Hiroaki Satoh, Akihiro Kudoh and Tsuyoshi Watanabe
Adipocytokines such as resistin, TNF-α, and adiponectin, which are adipocyte-derived peptides, play important roles in glucose metabolism. Resistin and TNF-α have been implicated as factors associated with the development of insulin resistance in obesity. In contrast, adiponectin has been shown to improve insulin sensitivity in insulin resistance. However, the interaction among these adipocytokines is still unknown. In this study, we investigated the mechanism of the effects of these 3 adipocytokines (resistin, adiponectin, and TNF-α) on glucose transport in 3T3-L1 adipocytes.
Glucose uptake was evaluated by 2-[3H] deoxy-glucose (DOG) uptake assay in 3T3-L1 adipocytes. Resistin and adiponectin secretion were analyzed by western blotting.
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