Mirto M, Randazzo V, Agueli C, Salemi D, Valenti D, Marfia A, Bica MG, Cannella S, Fabbiano F, La Rosa C, Caradonna F and Santoro A
Posters-Accepted Abstracts: Biol Syst Open Access
Acute Myeloid Leukaemia (AML) is frequently associated to normal karyotype and DNMT3A mutations (R882). Since we previously demonstrated distinctive miRNA expression in some AML groups, we study 384 miRNA in 9 selected DNMT3A-mutated NK-AML patients. Comparing these data with our previous results obtained in 31 DNMT3A-unmutated AML, we focused on a significant up-regulation of miR-155, miR-29a, miR-196b and miR-25. We investigated expression of these miRNAs in additional 24 DNMT3A-mutated AML patients and we confirm the up-regulation of miR-155, miR-29a and miR-196b; in particular, we judged very interesting the over expression of miR-29a since is known to directly target DNMT3A, TET1 and TDG mRNAs. Evaluating the expression levels of these targets in 17 AML DNMT3A-mutated patients, we revealed a no significant differences in expression of DNMT3A and TDG but a significant down-regulation of TET1. These data suggest that miR-29a acts as DNA methylation-regulator: in presence of DNMT3A activating mutations and TET1 down-regulation it may probably cause a perturbation of DNA methylation. In fact, analyzing the methylation of the bone marrow genomic DNA from 3 DNMT3A-mutated and 3 DNMT3A-unmutated cases by Methylation Sensitive Arbitrarily Primed-PCR, we found a genomic hypermethylation of DNMT3A-mutated cells compared to the unmutated ones. How DNMT3A mutations contribute to leukemogenesis is not yet well characterized. Uncovering how DNMT3A mutations affect DNA methylation and epigenetic regulation of gene expression may have important implications in treatment selection because DNA hypomethylating agents are increasingly used in AML therapies, and response to these drugs may be affected by DNMT3A changed function.
Maria Mirto is completing her master studies in Health Biology at University of Palermo (Italy). She worked and is working with a joint work group dedicated to Epigenetic studies in Acute Myeloid Leukemia consisting of Cellular biology and Genetics Lab of Department STEBICEF (University of Palermo) and Oncohematologic Integrated Diagnostic Lab (United Hospitals Villa Sofia Cervello Palermo) Italy. These teams have several publications in topic. These presented results are part of her degree thesis.
