Zhen-Hai Ma, Jin-Hui Ma, Li Jia and Yong-Fu Zhao
Posters: Surgery Curr Res
Objectives: By applying enhanced cDNA transfection in mice with hepatocellular carcinoma without metastatic cell lines (Hepa1-6) COL8A1, and observing cancer cell proliferation, invasion, tumor formation in vivo, this study aims at regulating the influence of COL8A1 expression on tumor cell drug sensitivity and tumorigenicity. Methods: The COL8A1 expression in transfected Hepa1-6 cells was analyzed with the use of RT-PCR, Western Blot. The transfection of ? Hepa1-6 cells of invasion ability ? was tested in invasive experiments in vivo and transfection of ?Hepa1-6 cells of the tumorigenic?ability was tested in vivo tumor. Transfection of Hepa1-6 cells proliferation and its effect on d-limonene susceptibility was analyzed using the method of MTT. Results: Expression of COL8A1 in experimental group cell of Hepa1-6 / COL8A1 showed a significant increase when compared to un-transfected cells of Hepa1-6 control group and empty plasmid transfected cells of Hepa1-6 / Mock control group. The ability of cell proliferation, matrix adhesion through the invasion and tumorigenic in vivo were enhanced and at the same time the cells of d-limonene susceptibility were down-regulated . Conclusion: COL8A1 expression in hepatocarcinoma cells in mice is related to tumor cell proliferation, invasion, in vivo tumorigenicity and antitumor drug sensitivity, which may provide new targets for tumor chemical therapy.
