Kalaivani Batumalaie, Rajes Qvist and ShamalaDevi Sekaran
Posters: J Diabetes Metab
Type2 diabetes is a chronic disease that is characterized by high glucose in the context of insulin resistance and relative insulin deficiency. It has been shown that oxidative stress might be implicated in promoting a state of insulin resistance and low-grade systemic inflammation. It is reported that reactive oxygen species (ROS) are involved in the progression of insulin resistance, which is characterized by impairment of glucose utilization and reduced insulin signaling in peripheral tissue. Oxidative stress is also known to be involved in the progression of β cell deterioration. This study investigates the antioxidant property of phenolic compounds extracted from local Gelam honey (Melaleuca spp.), on the production of ROS in hamster pancreatic cells (HIT-T15cells), which were cultured under normal and hyperglycemic conditions. Phenolic extracts from a local Malaysian species of Gelam honey (Melaluca spp.), was prepared using the standard extraction methods. HIT-T15 cells were cultured to confluence at 37?C in 5% CO2 and then pre-incubated with Gelam honey extracts (20, 40, 60 and 80?g/mL) as well as other phenolic compounds such as chrysin, luteolin and quercetin (20, 40, 60 and 80?M/mL), prior to stimulation by 20 and 50mM of D-glucose. The anti-oxidative effects were measured in these cultured cells at different concentrations and time points by 2?, 7?-dichlorfluorescein-diacetate (DCFH-DA) assay. The pre-treatment with local Gelam honey extract and phenolic compounds (chrysin, luteolin, quercetin) for 24 hrs resulted in a significant decrease in intracellular reactive oxygen stress (ROS) and an increase in the HIT-T15 cell viability. These results show the invitro antioxidative property of phenolic compounds from the Gelam honey against pancreatic β cells from hamsters.
Kalaivani Batumalaie has completed her Bsc Hons Biomedical Science at the age of 23 years from Management Science University, Malaysia. Currently she is preparing for her Master of Medical Science in the Faculty of Medicine, University of Malaya.
