Dhusia Kalyani, Yadav Arvind and Sagar Mamta
Posters-Accepted Abstracts: Biol Syst Open Access
Against Plasmodium, causative agent of malaria causing hundreds of millions of clinical infections and at least a million deaths per annum; a number of drugs have been designed but have encountered resistance, hence vaccination appears to be an efficient way for controlling spread of malaria. Rodent malaria parasite Plasmodium yeolii is considered because it reproduces many of the biological characteristics of the human malaria parasite whilst falciparum and vivax are particularly tricky as the complete life cycle cannot be maintained in vitro. Reverse vaccinology is an innovative approach to design efficient vaccines to overcome many difficulties of conventional vaccine development including culture of pathogen in lab that pose a chance to be hazardous. RV counts on the collective use of immunological and genomic information to recognize relevant protein antigens for vaccine development. Here in this work antigen determinants were predicted and MAPPP (MHC-I Antigenic Peptide Processing Prediction) made binding and proteasome cleavage prediction to identify possible antigenic peptides present on the cell surfaces. Molecule having greater LCV value was designed and showed potential to be a successful vaccine. This work demonstrated that Reverse Vaccinology approach is more likely to be discovering various immunogenic antigens from computational analysis of pathogenâ??s genome/proteome instead of culturing the whole organism by conventional methods.
