Aceng Ruyani, Agus Sundaryono, Dinisari Muthma?innah, Melly Br Sumatupang and Abas
Accepted Abstracts: Nat Prod Chem Res
Introduction: This research was aimed to study the potential of leaf ethanolic extract Etlingera hemisphaerica to recover testes and sperm quality in Mus musculus as the effects of mercury chloride (HgCl2) toxicity. Methods: Po, P1, P2, P3, and P4 group each consisting of 5 mice as first test animals were used. In day-1, P1, P2, P3, and P4 were given HgCl2, 5 mg/kg body weight (bw) by gavage, and then in days-3, 5, and 7, once a day, P2, P3, and P4 were administered extract 0.13, 0.26, and 0.39 mg/g bw by gavage respectively, meanwhile Po as the control received only the solvent. In day-16, the first test animals were killed by cervical dislocation, and dissected to determine weight, length, and width of the testes. Qo, Q1, and Q3 group each consisting of 15 mice as second test animals. In day-1, Q1 and Q2 were injected intraperitoneally HgCl2 5 mg/kg bw, then in day-3, Q2 was administrated extract 0.39 mg/kg bw by gavage, meanwhile Qo as the control received only the solvent. In day-6, the second test animals were killed by cervical dislocation, dissected, and generated suspension from the epididymis to determine sperm quality. Results: HgCl2 treatment on P1 increased weight, length, and width of the testes compared to Po, while HgCl2 treatment which was followed by extract administration three times on P2, P3, and P4 tended to restore testes similar to the Po. Extract 0.39 mg/kg bw on P4 was the most effective to recover testicular conditions. HgCl2 treatment decreased sperm concentration on Q1 (7.93x103/mL) compared to Qo (15.66x103/mL). While HgCl2 treatment which was followed by extract 0.39 mg/kg bw administration on P2 (11.16x103/mL) was lower than on Q1 and state approach Qo. Sperm moves fast and straight in Qo was 30.63%, Q1 was 16.12%, and Q2 was 27.62%, these facts indicated that extract 0.39 mg/kg bw could recover sperm motility due to HgCl2 toxicity. Conclusion: Leaf ethanolic extract E. hemisphaerica (0.39 mg/kg bw) is potentially to recover testes and sperm quality as the effects of HgCl2 (5 mg/kg bw) toxicity in M. musculus.
Aceng Ruyani has completed his PhD in developmental biology from Bandung Institute of Technology (ITB). He is the director of Magister Program in Science Education, Faculty of Teacher Training and Education, Bengkulu University, Bengkulu, Indonesia.
