Aisha M. K. Siddiqui, Rashmi S. Tupe1, Vaishali D. Mittal, Pavani S. Narayanam, Kamlesh B. Mahajan and Arundhati G. Diwan
Posters: J Diabetes Metab
To analyze possible influence of plasma amyloid on erythrocytes, 70 type 2 diabetic patients and 40 healthy subjects were recruited in this study. Erythrocyte oxidative damage was assessed for osmotic fragility, lipid peroxidation (LPO), reduced glutathione (GSH), catalase along with plasma amyloid content. Mean values were compared between diabetic and control groups and correlation were determined. Absorbance of plasma amyloid was significantly higher (p<0.001) in diabetic patients (0.53 ? 0.26 A530 nm) than controls 0.40 A530nm (p<0.001). Diabetic erythrocytes were extremely oxidatively damaged as LPO level was found to be significantly higher (p<0.001) in diabetics (0.11 nmol MDA/ml hemolysate) than in controls (0.074 nmol MDA/ ml hemolysate). The GSH (non enzymatic antioxidant) was also decreased in diabetic (101 nmol/ml) than controls (137 nmol/ ml) (p<0.001). However, catalase showed a minimal increase in diabetic erythrocytes (0.076 ? 0.061unit) as compared to controls (0.067 ? 0.043 unit). % hemolysis was also increased significantly (p<0.001) in diabetic patients (0.666 ? 0.073 MEF) as compared to control subjects (0.609 ? 0.096 MEF). Plasma amyloid content correlated with erythrocyte fragility (R = 0.130) and LPO (R = 0.151) indicating its toxic influence on erythrocytes. There was positive correlation between LPO and HbA1c, strong negative correlation of GSH and catalase with HbA1c levels. Our overall data suggests plasma amyloid content causes oxidative stress on erythrocytes in type 2 diabetic patients.
Aisha M. K. Siddiqui is a final year M. Sc. Biotechnology student doing her thesis / dissertation project under Dr. Rashmi Tupe at Rajiv Gandhi Institute of Information technology and Biotechnology, Bharti Vidyapeeth Deemed University Pune.
