Triana Hertiani, Maya Indra, Putri Khaerani and Sylvia Utami Tunjung Pratiwi
Scientific Tracks Abstracts: Nat Prod Chem Res
Myrmecodia tuberosa Jack (Rubiaceae) has been used in West Papua as part of traditional remedy with wide therapeutic value including as immune enhancer. Pseudomonas aeruginosa and Staphyoloccus aureus have been correlated with pathogenic opportunistic in immunesuppressed patient and this plant has been reported as a potential immunomodulator. Therefore research on this plant effect on the quorum sensing of these strains is worth exploring. Quorum sensing inhibition assay of the plant?s fractions was performed towards Pseudomonas aeruginosa inoculated on cetrimide agar. Samples in two fold dilution were prepared to gain 2-0.0625 mg/mL concentration. The effects on swimming, swarming and twitching motility of P. aeruginosa PAO1were recorded over control. All experiments were done in triplicate. Bioautography was performed on the extract in order to explore the active constituent. Obtained results suggested that the ethyl acetate fraction of M. tuberosashowed a prominent effect on quorum sensing inhibition of P. aeruginosa. Significant activities as quorum sensing inhibitor and anti motility were observed over control in a concentration dependent manner. Bioautography assay performed on the fraction showed no inhibition zone suggesting that quorum sensing inhibition may be a result of additive effect of compounds content. Nevertheless, phenolic compounds was identified as active fraction for planktonic growth inhibition. Myrmecodia tuberosa ethyl acetate fractionmight be developed as anti-infective against P. aeruginosa through inhibition of the microbe pathogenicity.
Triana Hertiani has completed her PhD degree from the Pharmazeutische Biologie und Biotechnologie Institute, Heinrich-Heine Universiteit, Duesseldorf, Germany, 2007. She is currentlythe Chief of Pharmaceutical Sciences Master Degree Program of the Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia. Her research interest is exploring Indonesian natural resources for antiinfective.
