Pooja Sharma, Arun M Gurav and Ajay Sharma
Accepted Abstracts: Nat Prod Chem Res
The major objectives of the present study were to develop an efficient micropropagation protocol and estimation of camptothecin (CPT) and 9-methoxycamptothecin (9-MCPT) content variation in regenerated plants of N. foetida. Callus culture was initiated from immature fruits of N. foetida, on Murashige and Skoog?s (MS) medium supplemented with different combinations of phyto-hormones. Maximum number of shoots (62.0 ? 0.02) and shoot elongation (33.0 ? 0.01 mm) was found in MS liquid medium supplemented with 4 μM of benzyl amino purine (BAP) and 8 μM of gibberellic acid (GA). Shoot elongation and number of shoots were relatively higher for explants incubated in MS liquid medium supplemented with 2.5 μM of Kinetin. The maximum number of roots (22.0 ? 0.01) and root elongation (33.7 ? 0.01 mm) was found in MS medium containing 4 μM of indole acetic acid (IAA) along with root induction of 94 percent. Hardening of plantlets was very successful with more than 90% survival. The CPT and 9-MCPT content was analyzed by high performance thin layer chromatography (HPTLC). The maximum CPT (2.86% w/w) and 9-MCPT (1.14% w/w) content was found in plants transferred in earthen pots where as minimum CPT (1.18% w/w) and 9-MCPT (0.47% w/w) content was found in callus. The proposed in vitro multiplicationprotocol can keep the pressure off from the wild population of N.foetida that can be used as an alternative source for the extraction of CPT and 9-MCPT to meet the global demand.
