Jian-ye Zhang, Hu-biao Chen, Tao Yi, Min-ting Lin and Si-li Tang
Scientific Tracks Abstracts: Nat Prod Chem Res
Objective: To observe the changes of mitochondrial membrane potential (MMP), cytochrome c (Cyto-c), Caspase-3, 9 activity and cleavage of PARP, and determine the apoptosis signaling pathway in Bruceine D -treatment K562 cells. Method: MTT assay was used to evaluate the cell growth inhibition of Bruceine D in vitro; Flow cytometry were performed to analyze MMP; Western Blot analysis was applied to detect Cyto-c, Caspases-3,-9 and PARP in K562 cells. Results: IC50 value of Bruceine D against K562 cells was 6.37 ? 0.39 μM. The percentages of MMP after the treatment of 3.0,6.0,12.0 μM Bruceine D for 24 h were 79.84 ? 4.46%, 59.74 ? 7.48%, 40.66 ? 4.37% (P<0.05) respectively. The release of Cyto-c, activity of Caspase-3, 9 and cleavage of PARP increased compared with the control groups in the Bruceine D induced K562 cell. Moreover, Bruceine D could decrease Phosphorylation level of AKT and ERK. Conclusions: The collapse of MMP, the increased Cyto-c, the up-regulation of Caspase-3, -9 activity and the augmented cleavage of PARP emerged after K562 cells treated by Bruceine D. The apoptosis of K562 cells induced by Bruceine D might be related to the mitochondrial pathway of apoptosis. Reduction of AKT and ERK Phosphorylation level might be mechanism of growth inhibition of K562 cells mediated by Bruceine D.
Jian-ye Zhang received BS of Pharmaceutical Sciences and MS of Pharmacognosy from Peking University, PhD of Oncology in Sun Yat-sen University. He has been a visiting scholar at School of Chinese Medicine, Hong Kong Baptist University for two years. He is currently Committee Member of the Society of Anti-Cancer Drugs, Chinese Anti-Cancer Association and Committee Member of Division of Tumor Pharmacology, Chinese Pharmacological Society.
