Haobo Li, Zipeng Liu, Aimin Xu, Chi Wai Chueng, Gordon T. Wong1, Michael G. Irwin and Zhengyuan Xia
Posters: J Diabetes Metab
I schemic heart disease (IHD) remains the leading cause of mortality and morbidity in diabetic patients. Ischemia postconditioning (IPostC) has been shown to be a effective way in combating myocardial ischemia-reperfusion injury, but the cardioprotective effects of IpostC is compromised or diminished in patients with diabetes, a metabolic disease that was associated with reduced levels of adiponectin (APN). Adiponectin (APN) is a protein that is secreted primarily from adipose tissue, which confers cardioprotection by enhancing myocardial nitric oxide production, a key mediator in IPostCcardioprotection, however, its role in IPostC mediated cardioprotection has not been investigated. The aims of the present study were to determine the role of APN in IPostC mediated cardioprotective effect and investigate the underlying molecular mechanisms. Wild-type (WT) and APN knockout (AKO) mice were subjected to 30 min coronary artery ligation followed by 2 hours of reperfusion, at the absent or present of IPostC achieved by 3 episodes of 10s reperfusion and 10s re-occlusion immediately after ischemia. Myocardial functions were assessed by pressure volume (PV) conductance system. Post-ischemic myocardial infarct size was higher in AKO relative to WT, which was associate with significant reduction of myocardial p-eNOS expression and end systolic PV relation, a reliable measure of ventricular systolic function, in AKO. In contrast, IPostC significantly reduce infarct size and improve end systolic PV relation, together with significant increase expression of myocardial APN, in WT but not in AKO. It is concluded that enhancement of myocardial APN may represent a key mechanism by which IPostC confers cardioprotection.
Haobo Li is currently a Ph.D. candidate in the University of Hong Kong, his research mainly focus on cardioprotection of postconditioning on myocardial ischemia reperfusion, particularly, of diabetic hearts. His work has been published in Journal of Diabetes & Metabolism, and Diabetes. Hehas orally presented his work at 8th International Symposium on Healthy Aging, and has been invited to present his work at the Experimental Biology 2013 conference at Boston, USA, April 2013.
