Dhara Jajal
Sardar Patel University, India
Posters-Accepted Abstracts: J Diabetes Metab
Diabetic retinopathy (DR) is a prominent micro-vascular complication of diabetes mellitus that affects as wide as 12 to 30% of the diabetic population in India. Vascular Endothelial Growth Factor-A (VEGF) plays a chief role in the pathogenesis of the DR. Many polymorphisms in the promoter and UTR regions of VEGF gene have been evidently shown to be correlated with development and progression of DR in different ethnicity. The present study investigated three intronic SNPs in VEGF gene that possibly predispose well-defined type 2 diabetes patients to DR or proliferative DR (PDR) in India; that were not previously studied in India. We performed PCR-RFLP to genotype the three SNPs rs833069, rs2146323 and rs3025021 in control healthy individuals, type 2 diabetic patients without DR (DWR) and type 2 diabetic patients with DR. The homozygous minor genotype (AA) of rs2146323 showed a potential risk to develop PDR (P<0.05, OR=5.44), whereas, it was not significantly associated with DR (OR=3.49). We found rs833069 polymorphism significantly associated with the susceptibility of DR and PDR, but its deviation from Hardy-Weinberg equilibrium made the results non-conclusive. Though, genotypic as well as the allelic distribution of rs3025021were not significantly different among DWR and DR patients, the odds ratio suggested TT genotype imparting possible risk to PDR (OR=4.27). In conclusion, our study indicated rs2146323 SNP as a potential genetic factor to influence the occurrence of PDR in the targeted Indian ethnicity.
Email: dhara.jajal@gmail.com
