Fang-fang Wang, Guo-Lian Ding and He-Feng Huang
Scientific Tracks Abstracts: J Diabetes Metab
The aim of this study is to investigate the mechanism of transgenerational transmission of glucose intolerance by intrauterine hyperglycemia associated with gestational diabetes mellitus (GDM). We established a mouse model of GDM and observed the phenotype of GDM off spring (F1-GDM). Furthermore, we intercrossed male and female F1 adult control (C) and GDM mice and investigated phenotype in F2 off spring. Mouse model of GDM was induced by a single intraperitoneal injection of streptozotocin on the day of pregnancy. We intercrossed male and female adult control (C) and fi rst-generation off spring of GDM (F1-GDM) mice to obtain the second-generation (F2) off spring in four groups: Câ??-C , Câ??-GDM , GDMâ??-C and GDMâ??-GDM . Intraperitoneal glucose tolerance tests were performed on 3-week-old and 8-week-old mice. Gene expression was detected by real-time quantitative PCR. (1) Compared with control group, birth weight increased in F2 off spring through the paternal line; (2) In F1-GDM, impaired glucose tolerance (IGT) appeared at adult mice. High risk of IGT appeared as early as childhood in F2 off spring and progressed through both parental lineages, especial paternal line; (3) IGT in both F1 and F2 generations is associated with abnormal Igf2/H19 expression in islet. During pregnancy, intrauterine hyperglycemia environment of GDM lead to IGT in both fi rst- and second-generation off spring. Abnormal expression of Igf2/H19 in islet may be one of the mechanisms for transmission of glucose intolerance induced by intrauterine hyperglycemia.
Fang-Fang Wang has completed her bachelor degree with the major of Clinical Medicine in Zhengjiang University from 2003 to 2008; Then master degree of Clinical Medicine in the fi eld of obstetrics and gynecology from 2008 to 2010; and she is in the 2nd year of her Ph.D program with the major of reproductive endocrinology. She has 3 papers published on the top journal in the reproductive endocrinology fi eld and 2 papers submitted to the journal of DIABETES and JMM.
