Chandan K. Maurya, JyotsnaPandey, Natasha Jaiswal1,T. Narender and Akhilesh K. Tamrakar
Posters: J Diabetes Metab
nsulin resistance, defined as the inability of insulin to stimulate glucose uptake in insulin sensitive tissues, such as the skeletal muscles and adipose tissue, plays crucial role in development of type 2 diabetes (T2D) and metabolic syndrome. Skeletal muscles are the principal site for postprandial glucose utilization and major candidate to maintain glucose homeostasis. Insulin resistance in skeletal muscle is characterized by impaired translocation of insulin sensitive transporter-4 (GLUT-4) and consequent defect in insulin-stimulated glucose uptake, a rate limiting step for glucose disposal. Thus modulating GLUT-4 translocation provides a novel approach to combat insulin resistance and the progression of T2D and other associated metabolic complications. Therapeutic approaches with natural products provide a fruitful source for searching safe, effective and relatively inexpensive new remedies for diabetes mellitus and associated metabolic disorders. In continuation of our search towards natural molecules with GLUT-4 translocation modulatory effects, standardized fraction of Peganumharmala (F015) was evaluated for GLUT-4 translocation and glucose uptake stimulatory effect in skeletal muscle cell lines under in vitro culture conditions. The F015 was found to enhance both glucose uptake and GLUT-4 translocation to cell surface in a concentration-dependent fashion. Further evaluation of the effects of F015 on insulin signalling pathway suggests that it act as an insulin-mimetic agent and stimulates glucose uptake in a PI-3-K/AKT dependent mechanism. Our findings indicate that F015 may have potential for developing new molecules for the treatment of insulin resistance and associated metabolic disorders.
I am Chandan Kumar Maurya, a research scholar at Central Drug Research Institute, Lucknow. Presently I am working on type 2 diabetes. I have completed M.Sc. in Biochemistry from V.B.S. Purvanchal University,Jaunpur, Uttar Pradesh there after qualified the CSIR ?NET JRF fellowship. I have 1 research Publication.
