Akanksha Bhargava, Nazia Afreen, Neena Sankhe, Devyani Phatak, Juhi Parikh and Rashmi Tupe
Posters: J Diabetes Metab
Hyperglycemia is a widely accepted participant in the development, progression and complications of diabetes. Glycation of proteins leading to partial loss of activity is one of the serious consequences of chronic hyperglycemia. In present study aqueous extracts of four nutraceuticals, namely, Laurus nobilis (Leaf), Syzygium alternifolium (Seed), Phaseolus vulgaris (Seed) and Mentha arvensis (Leaf) were prepared at the concentration of 10mg/ml. The measurements of antiglycation potential of these nutraceuticals were done by performing the antiglycation assays such as estimation of protein carbonyl group, thiol, binding of Congo red, fructosamine adduct, phenyl borate affinity chromatography and protein electrophoretic pattern. Protective effect of these nutraceuticals against glycation induced cytotoxicity in renal cells (HEK-293 cell line) was assessed by estimating % viability, reduced glutathione and total antioxidant capacity as FRAP levels. In vitro glycation assays demonstrated that nutraceuticals exerted site specific inhibitory effects at multiple stages. P. vulgaris showed good results by alleviating carbonyl stress, free thiol concentration by 23.78%, 52.58 % respectively and S. alternifolium reduced fructosamine and amyloid content by 2.70%, 81.77 % which was observed in affinity chromatography. In cellular studies, renal cells (HEK 293) pretreated with S. alternifolium showed maximum resistance to glycated protein toxicity (higher viability, reduced glutathione and FRAP levels) with normal cellular morphology. Overall results indicated that S. alternifolium prevented glycation induced protein modifications and protected renal cells thus merit further investigation of its activity
Akanksha Bhargava is a final year M. Sc. Biotechnology student doing her thesis / dissertation project under Dr. Rashmi Tupe at Rajiv Gandhi Institute of Information technology and Biotechnology, Bharti Vidyapeeth Deemed University, Pune.
