Andleeb Batool
Government College University, Pakistan
Posters & Accepted Abstracts: J Diabetes Metab
Type-2 diabetes (T2D) has been the subject of numerous genetic studies in recent years which revealed associations of the disease with many susceptibility loci. We evaluated 10 genes (TCF7L2, HHEX, ITGA2, CDKL1, ADRB3, PRKCB1, CETP, GNB3, LTA, LPL and PPARG) for susceptibility of T2D in Pakistani population by performing a case-control study. A total of 350 subjects (Patients=280 and Controls=70) participated in the study. Genotyping was performed by PCR-RFLP and overall 6 SNPs from the study were found to be significantly associated with T2D. ITGA2 (rs1062535-rs1139484-KT359366) was first time studied in south Asians which showed a significant association with T2D and a novel SNP was identified and got its accession number (KT359366). The loci from HHEX, ITGA2, LTA, PPARG and ADD1 remain associated with T2D after SNP and genotype analysis (P<0.05) while GNB3 and PRKCB1 were only associated in genotype analysis. Two haplotypes (GAT, GGC) from ITGA2 were associated with T2D development while GGT (p-0.0001) was protective against diabetes. No association was detected with TCF7L2, CDKL1, ADRB3, CETP, GNB3, and LPL. This data can be used for prevention and screening of population at risk, moreover can be helpful in large scale studies.
