Eduardo Jose Caldeira, Victor Augusto Ramos Fernandes, Jefferson Benedito Nery, Ana Carolina Morais, Gisele Caetano, Tulio Liboni Bc, Nilva Karla Cervigni and Taize Augusto Machado
Tissue Morphology Laboratory, Brazil
Posters & Accepted Abstracts: J Diabetes Metab
NF-�ºB is a protein involved in the pathogenesis of diabetes and the progression of this disease. Different studies show that this protein is elevated in this condition, also contributing to the development of oxidative stress and inflammation, which further potentiates the harmful effects of hyperglycemia. Different organs and tissues suffer with the diabetes, mainly the parotid salivary gland, due to its protein characteristic, which seems increase further these damages. The aim of this study was to verify if the use of alpha tocopherol decreases the expression of NF-�ºB protein and reduces the damage in parotid salivary cells submitted to hyperglycemic condition. Twelve BALB/c mice, 5 weeks years-old, were sacrificed and cells were removed from the parotid salivary glands. After extraction, the Percoll gradient protocol was applied for the cellular isolation. The cells were then incubated with different glucose doses, being 2.0g/L for the control group (I) and 4.5g/L for the hyperglycemic group (II), and treated group with 4.5g/L plus 20uM alpha tocopherol (III). After the 16 hour exposure, cells were submitted to cell viability assay (MTT) and protein quantification (western blotting). The present study was approved by Ethics Committee of the Faculty of Medicine of Jundiai (154/2016). Hyperglycemic groups, with 4.5 g/L of glucose and 4.5 g/L of glucose with 20 �¼M of alpha tocopherol presented lower cell viability when compared to the control group. Also, these groups showed higher expression of NF-�ºB when compared to control group (I). The group treated with alpha tocopherol (III) did not present statistical differences compared to the untreated hyperglycemic group (II). Hyperglycemia induces lower cell viability and, in particular, a greater expression of NF-�ºB in parotid salivary cells, which may demonstrate a potentiation of the damage caused by diabetes. Thus, in vitro, alpha tocopherol was not effective in reversing this condition.
Eduardo Caldeira has his expertise in type 1 diabetes and treatments. He use in vivo/in vitro diabetes model to test new approaches to reverse the harmful effects caused by the hyperglycemic condition.
Email: drdcaldeira@gmail.com
