Ikpefan Emmanuel Oise
Delta State University, Nigeria
Posters & Accepted Abstracts: Nat Prod Chem Res
Bioassay guided fractionation of the methanol extract of Euphorbia graminea against breast cancer (MCF-7), lung cancer (NCI-H460) and NIH 3T3 (mouse embryonic fibroblast normal cell line) at 1-250 μg/mL was carried out. Extracts of E. graminea was partitioned into aqueous and chloroform fractions and both fractions were tested for their effects on MCF-7 and NCI-H460. Further chromatographic and biological studies of the active chloroform fraction yielded a compound whose identity was revealed as Abietane-11, 23 diene-16-oic-14-ones through NMR and MS studies. This compound was observed to give â??3.3±1.4 and 5.30±3.75% cytotoxicity against MCF-7 and NCI-H460 at 100 μM with GI50 and TGI of +38±0.74, 96.94±6.95 μM and 53.70±9.30 and 93.88±11.70 μM respectively. The result has established the rationale for the use in ethnomedicinal practice.
Email: ikpefanemmanuel@delsu.edu.ng
