Jasia Bokhari and Muhammad R Khan
Accepted Abstracts: Nat Prod Chem Res
The aim of present study was to investigate the cardioprotective effect of Digera muricata (L) Mart. against the cardiotoxicity induced by acrylamide in rats. Forty eight healthy female albino rats, weighing 190-200 g, divided into eight groups with six rats in each, group I as control, while group II were administered with dimethylsulphoxide (DMSO) 5.0 ml/kg b.w. orally once a day for four weeks. Group III was given 200 mg/kg b.w. of methanolic extract dissolved in DMSO once a day for four weeks. Rest of the rats were divided into five groups and were treated with aqueous solution of acrylamide 6 mg/kg b.w. intraparetoneally once a day for two weeks. Group IV was sacrificed after 15 days of the acrylamide treatment to collect the serum and heart gland. Group V remained untreated as such for the rest of the experiment Group VI, VII and VIII were given 100, 150 and 200 mg/kg b.w. methanolic extract dissolved in DMSO once a day for two weeks. After four weeks all the animals were disected and the blood was collected by cardiac puncture and 5-7 ml blood was taken in falcon tube and was centrifuged to collect the serum and stored at -20?C for biochemical studies. Co-treatment with Digera muricata extract at 100, 150 and 200 mg/kg prevented the elevation of serum marker enzymes creatinine kinase (CK), cardiac creatinine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), hydrogen peroxidase (H2O2), total cholesterol, LDL and alterations in protein, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione (GSH), glutathione-S-transferase (GST), Gluthaione peroxidase (GSHPx), γ-glutamyl transpeptidase (γ- GT), HDL, creatine and urea caused by acrylamide in rats. The protective effect was confirmed by the histological findings and was more prominent at 200 mg/kg. Hence we conclude that methanolic extract of Digera muricata protects against acrylamide induced cardiac toxicity.
