Mohamed Hemida Abd-Alla, El-Sayed A El-Sayed, Abdel-Hamied M Rasmey, Ismail A Kady and Ibrahim M Yassin
Posters-Accepted Abstracts: Biol Syst Open Access
Most immunosuppressive agents were initially developed as antibiotics produced by the genus Streptomyces. This study was devoted to examine the antimicrobial activity of the Streptomyces variabilis ASU319 extract and testing the purified active compound of this extract as immunosuppressive agent in rats� blood. Elucidation the chemical structure and optimization of the active compound were studied as well. Antimicrobial activity was conducted using agar-well diffusion and disc diffusion assays. The antimicrobial metabolite was extracted from the fermentation broth by ethyl acetate and purified by TLC and silica gel column chromatography. The pure active compound was then subjected to spectroscopic analyses: 1H NMR, Elemental analysis, IR and Mass spectra. The active antimicrobial compound was tested as an immunosuppressive agent by injection in the rat blood and the complete blood picture (CPC) was determined. The crude extracts of the selected active antagonistic five isolates were tested to prevent the inflammation and proliferation of lymphocytes of the rats� blood. The active antimicrobial compound of Streptomyces ASU319 was purified and tested as immunosuppressive agents. The tested compound decreased each of the neutrophils, lymphocytes and monocytes than the positive control. The compound was of molecular weight 458-g/ mol and had given the proposed chemical formula C24H46O8. The producer isolate was identified by 16SrRNA sequencing as Streptomyces variabilis ASU319 with accession number [GenBank: KC145278]. These results demonstrated that Streptomyces variabilis ASU319 is potential microbial isolate for active antimicrobial compound production and has the ability to decrease the proliferation of the lymphocyte cells in the blood and may be a good immunosuppressive agent.
