Neeraj K Patel and Kamlesh K Bhutani
Accepted Abstracts: Nat Prod Chem Res
In the continuous search for newer anti-inflammatory leads from natural products, many plant extracts have been tested for their inhibitory effects towards pro-inflammatory cytokines. Among them, ethyl acetate fraction (MPE) of M. pudica whole plant have demonstrated the significant inhibition of NO, TNF-α and IL-1β production in RAW 264.7 cells with an IC50 of 34.4, 31.7 and 47.2 μg/mL respectively. Phytochemical investigation of the active ethyl acetate (MPE) fraction of M. pudica(L.) leaves yielded fourteen compounds. Among them, L-mimosine (IC50=19.23 to 21.15 μM), crocetin (IC50=23.45 to 25.57 μM) and its ester crocin (IC50=27.16 to 31.53 μM) and jasmonic acid (IC50=29.42 to 21.32 μM) were discovered as potent NO inhibitor when tested on the both cells. Towards TNF-α and IL-1β inhibition, ethyl gallate, crocetin, crocin, gallic acid, L-mimosine, jasmonic acid and caffeic acid were found to be more active with half maximal concentration, 17.32 to 62.32 μM whereas the other compounds depicted moderate and mild effects (IC50= 59.32 to 95.01 μM). Results suggested that these compounds imparting greatly to anti-inflammatory effects of M. pudica in vitro through reduction of LPS-induced pro-inflammatory mediators which affirm the ethno-pharmacological use of this plant for prevention of inflammatory-related disorders.
