Shahnaz Jalil, Prawej Ansari, S Azam, Gazi Md. Mustakim, F F Archi and J M A Hannan
North South University, Bangladesh
Posters & Accepted Abstracts: J Diabetes Metab
Hibiscus rosa-sinensis (HRS) has formerly demonstrated anti-hypoglycemic activity in type 2 diabetic rats. However, study on organ and tissue level has not been done yet. Our study is to find out, whether the antidiabetic activity is the result of inhibition of the action of gastrointestinal enzyme or decrement glucose absorption. For the investigation of hypoglycemic activity at primary stage, we studied fasting blood glucose and measured glucose tolerance test, in type 2 diabetic rats. Serum glucose, lipids, plasma insulin, insulin level in pancreases and hepatic glycogen content were assayed in this study. Sucrose malabsorption was evaluated in the six different parts of GI tract. Effect of extract on glucose absorption in the gut was studied by an in situ intestinal perfusion technique. The extract showed significant (p<0.05) anti-hyperglycemic activity at fasting as well as postprandial state in the glucosefed type 2 diabetic rats at 120 min and 240 min. During sucrose malabsorption test, unabsorbed sucrose content in gut was found to be higher in comparison to control and this test was confirmed by in situ intestinal perfusion technique, where extracts remarkably inhibited the glucose absorption in GIT tract. In chronic study, HRS significantly decreased serum glucose (P<0.05), Cholesterol (P<0.05), TG (P<0.01) and increased HDL (P<0.01) and the hepatic glycogen (P<0.05) without significant increase in body weight and enhanced circulating insulin. Hypoglycemic activities of HRS in type 2 diabetic rats were mediated by decrease in carbohydrate absorption from the gut. This combination of in vitro, in vivo and in situ tests established the anti-hyperglycemic activity of H. rosasinensis and its tissue oriented mechanism.
Email: shahnaz.biva@gmail.com
