Sumi Wijaya, Ting Kang Nee, Khoo Teng Jin and Christophe Wiart
Accepted Abstracts: Nat Prod Chem Res
The hexane, ethyl acetate and ethanol extract of Mikaniascandens (L.) Willd.were assessed for their antibacterial, antioxidant, anti-inflammatory and acetylcholinesterase inhibitory activities. The antibacterial activity was evaluated by the pour-plate disc diffusion assay, the minimum inhibitory concentration, the minimum bactericidal concentration and the death kinetic assay. The antioxidant activity was evaluated by the ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching assays. The anti-inflammatory activity was determined by 5-lipoxygenase inhibitory activity (5-Lox), meanwhile acetylcolinesterase activity was determined by the TLC-bioautography assay. Among the extracts, the hexane extract showed the best antibacterial properties. The ethanol extract showed the highest antioxidant and good 5-lipoxygenase inhibitory activity with an IC50 value equal to 23.87 μg/ml. The best acetylcholinesterase inhibitory activity was elicited by the hexane and ethanol extracts. The total phenolic content values were in the range 106.67 mg/g to 1066.67 mg/g gallic acid equivalent in the Folin-Ciocalteu assay. The results suggest that Mikaniascandens (L.) Willd.may provide a natural source of antibacterial, antioxidant, anti-inflammatory and acetylcholinesterase agents.
Sumi Wijaya works in Widya Mandala Catholic University, Indonesia. She has keen interest in bio constituents from Natural Source.
