Department of Nephrology, West China Hospital, Sichuan University, Chengdu, China
Research Article
PGC-1α and p38 MAPK are involved in uncoupling protein 2-dependent reactive oxygen species production in renal tubular epithelial cells
Author(s): Xiaolei Chen, Xiaoyu Xie, Siyu Duan and Chunlai Nie*
Object: The objective was to investigate Uncoupling protein 2 (UCP2)-dependent Reactive oxygen species (ROS)
production in renal tubular epithelial cells treated with high glucose.
Methods: In this study, we investigated the associations among PGC-1α, p38 MAPK and UCP2 in HK-2 cells
treated with high glucose. Target genes overexpression and silence were obtained by plasmid transfection, and
SB203580 was applied as a p38 MAPK inhibitor. The mutual effect of these three proteins was evaluated by RTPCR
and western blot. ROS production was detected by a fluorescent probe.
Results: We found that UCP2 overexpression enhanced PGC-1α expression, as well as inhibited p38 MAPK
activation and ROS generation. These changes were reversed by UCP2 siRNA. However, neither PGC-1α nor p38
MAPK could e.. View More»
DOI:
10.35248/2472-1220.20.6.191
