Department of Food Chemistry and Functional Nutrition, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan
Research Article
Glucosinolate Sinigrin Improves Insulin Resistance to Suppress Glutathione Consumption in Type 2 Diabetic Mice
Author(s): Thao Thi Phuong Truong and Tomoyuki Koyama*
Background: Sinigrin, an aliphatic glucosinolate, is absorbed from the intestine as allyl isothiocyanate (AITC) and conjugated with glutathione (GSH) followed by excretion as an N-acetylcysteine (AITC-NAC) into the urine. AITC is the crucial metabolized form which reflects the occurring bioavailability of dietary sinigrin. However, whether the anti-diabetic effects of dietary sinigrin and the quantitative of metabolic parameters AITC and AITC-NAC remain unknown in the type 2 diabetes model (T2D).
Methods: A total of mice were divided into 6 groups: (i) normal control (ii) diabetic control, (iii) normal + 15 (μmol sinigrin/kg BW for all), (iv) diabetes + 15, (v) normal + 30 and (vi) diabetes + 30. After oral administration of sinigrin for 21 days, plasma, tissue, and urine were collected for analysis of metabolic parameters.
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