Department of Chemistry, Lahore Garrison University, Lahore, Punjab, Pakistan
Research Article
Isolation of Natural Caffeine From Lipton™ Black Tea Through Acid-Base Liquid-Liquid Extraction Approach, Its Medical Significance and Its Characterization By Thin Layer Chromatography and IR Analysis
Author(s): Manzar Zahra*, M.Fazal-ur-Rehman and Iqra Qayyum
To isolate the caffeine from Lipton? Black Tea Brand, a sequence of practices was applied. An Acid-Base LiquidLiquid Extraction approach carried out to force the caffeine to be isolated in upper organic layer as extractant. By this
method, first caffeine was isolated from tea bags through passing different steps of extraction, then caffeine was
isolated from tea with the use of both Solid-Liquid approach as well as liquid-liquid extraction approach. A product
of 0.05 g of pure caffeine was obtained giving the percentage yield or percent recovery of 1.22%. Calculated percent
recovery was 1.2 %, this percentage yield clarified that in this tea brand, very small caffeine is investigated, this
deduces a significant loss of product throughout the procedure which are due to formation of emulsions and not due
to washing thoroughly with DCM to extract maximum yield. It is also significant .. View More»
