Editorial Office, Steroids and Hormonal Science, Germany
Short Communication
Boldenone and Related Steroids are Formed in The Faeces of Veal Calves
Author(s): Jennifer Stewart*
The use of NBoldenone as a growth promoter in meat-producing animals is prohibited in the European Union (Directive 96/22/EC). Boldione (ADD), boldenone, and boldenone esters (primarily the undecylenate form) are all commercially available as anabolic preparations for humans, horses, and cattle. Boldenone metabolites have been discovered naturally in cattle since the late 1990s. According to EU regulations, an unambiguous demonstration of boldenone administration in bovine urine must be provided based on the identification of boldenone in the corresponding conjugate fraction. A method for measuring intact 17-boldenone-sulphate has been developed and validated in accordance with current standards.
The analytical procedure included direct extraction–purification of the target analyte on octadecylsilyl cartridges, as well as direct detection of the phase II metabolite via.. View More»