College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
Research Article
HPLC Method for Determination and Optimization of 2,4-DNPH PreColumn Derivatization Effect of Reaction Conditions for Muscone in Natural Musk
Author(s): Myadagbadam U, Meng GD, Wang Y, Hasi S, Erdenechimeg CH* and Chimedragchaa CH
To establish a method for the determination of muscone in natural musk by pre-column derivatization HPLC with 2, 4-Dinitrophenyl hydrazine (DNPH) and the reaction conditions were optimized. The influencing factors of derivatization reaction were investigated by single factor, and the main influencing factors were optimized by orthogonal test. Under the optimum reaction conditions, muscone-DNPH derivatives were obtained and detected by 365 nm following the Alltima C18 column (5 μm, 150 mm × 4.6 mm) separation. Single factor investigation revealed: the suitable reaction medium and catalyst were ethanol and hydrochloric acid, respectively; the molar ratio of muscone to DNPH should not be less than 300 times, the appropriate reaction time was 20 min or more; the effect of reaction temperature on derivatization reaction was obvious and needs to be optimized. Furthermore, the ortho.. View More»
