Genetic Engineering and Biotechnology Institute, University of Baghdad, Iraq
Short Communication
Molecular Versusconventional Diagnosis Of Neisseria Gonorrhoeae
Infection And Study Its Role In Azf Locus Microdeletions
Author(s): Ghazwan A. M. Al-Rammahi*, Ismail A. Abdul-Hassan and Ashwak B. Jassim
Eighty two clinical samples were collected from suspected gonorrhoeae-infected patients (urethra swab and blood
sample were taken from each patient). During a period from the beginning of December 2012 to the end of
April 2013. In Al- Yarmouk teaching hospital (Baghdad, Iraq) and private laboratories, all of the patients were
married and have children compared with 20 samples been taken from apparently healthy control. All samples
were bacteriologically examined by traditional methods for detection of N. gonorrhea, 82 isolates were identify by
microscopic examination,76 isolates on Modified Thayer martin media and 61 isolates by PCR technique targeting
Orf1 gene. The results revealed that all samples which give positive results with Orf1 gene yielded negative results
for both SY 254 (85pb), BPY-2(90pb) genes. In the present study show no correlation between Y chro.. View More»
