Department of Biotechnology, Guru Nanak Dev University, Amritsar, India
Short Communication
An Extensive Examination of Non-Canonical Proteins in Non-Small Cell Lung Cancer and Their Effects on the Immune System
Author(s): Deepti Pathak*
Neoantigen mining is of great interest for cancer applications. Neoantigens in cancer have been found to be frameshift-mutated non-canonical proteins. We looked at the Non-Canonical Protein Landscape in Non-Small Cell Lung Cancer (NSCLC) and how autoantibodies were produced as an immune response. All alternate Open Reading Frames (altORFs) and ORFs discovered in pseudogenes, noncoding RNAs, and untranslated sections of mRNAs that did not align with known canonical proteins were compiled into a database of cryptoproteins. To determine the presence of cryptoproteins, the proteomic profiles of seventeen Lung Adenocarcinoma (LUAD) cell lines were examined. Plasma Immunoglobulin (Ig)-bound cryptoproteins were analyzed by mass spectrometry to determine the immunogenicity. The specimen collection included 102 control plasmas, pre-diagnostic plasmas from 51 NSCLC cases, and plasmas from 30 ne.. View More»
