Department of Forensic, Denmark
Perspective
High-throughput Sequencing (HTS) and DNA Fingerprinting (T-RFLP) are Being Compared to Determine the Richness and Makeup of Microbial Communities in Groundwater Ecosystems
Author(s): Alexander Wilson*
Most of the available freshwater is underground as groundwater. Pure groundwater ecosystems are classified as oligotrophic settings because they support a variety of well adapted species with limited energy yield, activity, growth, and reproduction. Thus, structural changes in groundwater species communities reflect declines in groundwater quality and quantity. Despite growing awareness of this issue, the only basis for current assessment methodologies for groundwater ecosystems is the evaluation of abiotic characteristics. However, to identify changes in microbial communities and their associated metabolic processes, this technique is insufficient. Our understanding of the diversity and makeup of microbial communities in various contexts has significantly improved in recent years as a result of the advent of culture-independent molecular tools to investigate bacteria. By allowing the.. View More»
DOI:
10.35248/ 2684-1312.23.8(2).1-2
