Mohammad Saadati* and Mostafa Mirzaei
Many digestive enzymes were reported from the gut and salivary gland of sunn pest, Eurygaster integriceps. Lipase and amylase are two main enzymes that play key role in the lipid and carbohydrate metabolism. Enzyme activities are affected with biotic and abiotic factors in the in vivo and in vitro condition. Nowadays, digestive enzymes– insecticides interaction in the insect body is not clear. In this study, the effects of four insecticides, cypermethrin, chlorpyrifos, diazinon and deltamethrin, on the lipase and amylase activity were determined. Our analysis revealed that lipase was more sensitive to insecticides in comparison to the α-amylase. Cypermethrin have showed the maximum inhibitory effects on the amylase and lipase activity in comparison to the others compounds. Chlorpyrifos and diazinon have inhibited digestive enzyme activities in the similar level. Results indicate that lipase and α-amylase are very sensitive to insecticides and were profoundly inhibited by cypermethrin, chlorpyrifos, diazinon and deltamethrin. Accordingly, metabolism of carbohydrates and lipids in the gut of sunn pest will reduce and nutrition process may be suppressed.
