Finding and recovering biological evidence that can be used to establish a connection between the offender and the complainant is one of the main goals of forensic investigation into sexual offences. The use of an Alternate Light Source is one potentially useful technique by which trace biological evidence may be recognised in different forensic circumstances (ALS). The purpose of this study was to ascertain whether or not employing an ALS as an adjunct during sexual assault examinations could help with the identification of forensically important body parts that are difficult to identify during visual inspection for sample. We provide two case reports that demonstrate the potential benefit of utilising an ALS as an adjunct in clinical forensic medical practise. sexual assault examinations to find regions of skin that could be forensically useful to sample for semen. We conducted a number of quick laboratory studies to establish a methodology for the use of the ALS before integrating it into our clinical forensic medical practise. Semen has been observed to glow using an ALS at 450 nm. Even if we didn't carry out a careful We tested the method's effectiveness using an ALS to find semen on a variety of inanimate objects and people skin. Reduced light source distance from the surface and increased semen concentration on the surface increased the visibility of fluorescence on all surfaces, but the angle at which the light source was held in relation to the surface had no discernible impact.
